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            1. 產品資料
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              Neuro-2a 小鼠腦神經瘤細胞

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              產品名稱: Neuro-2a 小鼠腦神經瘤細胞
              產品型號: CCL-131
              產品廠商: 美國ATCC
              產品文檔: 無相關文檔


              簡單介紹

              CCL-131 Neuro-2a 小鼠腦神經瘤細胞,原代細胞|細胞系|細胞株|菌種;細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和Z優培養條件!


              Neuro-2a 小鼠腦神經瘤細胞 的詳細介紹
              CCL-131 Neuro-2a  小鼠腦神經瘤細胞
              ATCC® Number: CCL-131?     Price:

              Designations: Neuro-2a
              Depositors:  RJ Klebe
              Biosafety Level: 1
              Shipped: frozen
              Medium & Serum: See Propagation
              Growth Properties: adherent
              Organism: Mus musculus (mouse)
              Morphology: neuronal and amoeboid stem cells
              CCL-131 Neuro-2a 小鼠腦神經瘤細胞
              Source: Strain: A
              Organ: brain
              Disease: neuroblastoma
              Cell Type: neuroblast;
              Cellular Products: acetylcholinesterase
              tubulin
              Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
               
              Applications: transfection host (Nucleofection technology from Lonza
              Roche FuGENE® Transfection Reagents)
              Virus Susceptibility: Herpes simplex virus
              Vesicular stomatitis virus
              Human poliovirus 1
              Antigen Expression: H-2, a haplotype; Mus musculus, expressed
              Cytogenetic Analysis: modal number = 95; range = 59 to 193.
              Karyotype unstable within a stemline range of 94 to 98 chromosomes. All the cells contain 6 to 10 large chromosomes with median or submedian centromeres and 2 to 4 minute chromosomes.
              GenoType: albino
              Comments: Clone Neuro-2a was established by R.J. Klebe and F.H. Ruddle from a spontaneous tumor of a strain A albino mouse. This tumor line, designated C1300, was obtained from the Jackson Laboratory, Bar Harbor, Maine [22161].Neuro-2a cells produce large quantities of microtubular protein which is believed to play a role in a contractile system which is responsible for axoplasmic flow in nerve cells.The cell line has been used for studies on the mechanism of vinblastine precipitation of microtubular protein, the kinetics of GTP binding to isolated protein, the turnover of microtubules in vivo, and the synthesis and assembly of microtubular protein [PubMed: 5263744]. The World Organization for Animal Health (OIE) uses the cells for routine diagnosis of rabies. (see: http://www.oie.int/Eng/Normes/Mmanual/A_00044.htm)Tested and found negative for ectromelia virus (mousepox).
              Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
              Atmosphere: air, 95%; carbon dioxide (CO2), 5%
              Temperature: 37.0°C
              Subculturing: Protocol:
              1. Remove and discard culture medium.
                • Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
                  • Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
                    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
                    • Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
                      • Add appropriate aliquots of the cell suspension to new culture vessels.
                        • Incubate cultures at 37C.

              Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
              Medium Renewal: 1 to 2 times per week
              Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
              Storage temperature: liquid nitrogen vapor phase
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